Table 1.

HinfI digestion of semi-nested PCR products

		Mutated
	No digestion	Heterozygous	Homozygous/LOH?	Wild-type
189 bp	+	–	–	–
121 bp	–	+	+	+
68 bp	–	+	–	+
54 bp	–	+	+	–
14 bp*	–	+	+	–

*Not visible on a polyacrylamide gel.

LOH, loss of heterozygosity.