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Epstein-Barr virus (EBV) genetic analysis in Escherichia coli: construction of the mini-EBV plasmid. Fragments from the EBV genome were sequentially added to the F-plasmid backbone using the chromosomal building technique. This mini-EBV carries all the latent genes and can immortalise primary B cells after encapsidation in a helper cell line or direct transfection of the plasmid. EBNA, EBV encoded nuclear protein; LMP, latent membrane protein.
