Figure 1.

Detection of human ccn3 (nov) mRNA in human prostatic cell lines by reverse transcription polymerase chain reaction (RT-PCR). A 1 μg aliquot of reverse transcribed RNA was subjected to PCR with specific primers for ccn3 (nov) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). PCR products were run on a 1.8% agarose gel and stained with ethidium bromide. Colours have been inverted for better clarity. Results shown represent a typical experiment.