Figure 1.

Non-denaturing polyacrylamide gel electrophoresis comparing the Taq polymerase amplified Bat-26 marker and individual clones obtained after subcloning this PCR product. The original PCR product (indicated by the arrow) was a pool of molecules with different repeat lengths and it migrates as a blurred area composed of “shadow bands”. In contrast, cloned individual molecules containing a known number of repeated adenines (indicated by numbers) are seen as discrete bands. The individual clones contain an extra 11 bp of vector sequence after restriction digestion of the cloned insert with EcoRI and thus migrate more slowly than the original PCR product.