Table 1.
Repeat length of individual DNA sequences subcloned from a PCR product pool after amplification of mononucleotide or dinucleotide repeat markers
| Locus | Repeat | Repeat length and corresponding number of clones | N | % Correct | |||||||||
| RAC1 | (T)9 | 9 | |||||||||||
| (Taq) | 10 | 10 | 100 | ||||||||||
| RAC1 | (T)11 | 10 | 11 | ||||||||||
| (Taq) | 1 | 9 | 10 | 90 | |||||||||
| Bat–13 | (T)13 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | |||||
| (Taq) | 9 | 8 | 20 | 19 | – | 1 | 1 | 58 | 33 | ||||
| (Pfu) | – | – | 2 | 16 | 1 | – | – | 19 | 84 | ||||
| Bat–21 | (T)21 | 16 | 17 | 19 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | ||
| (Taq) | 1 | 1 | 2 | 1 | – | 6 | 5 | 19 | 3 | – | 29 | 3 | |
| (Pfu) | – | – | – | – | 2 | 2 | 2 | 7 | 3 | 2 | 19 | 0 | |
| Bat–21 | (TA)11 | 6 | 9 | 10 | 11 | 12 | 13 | ||||||
| (Taq) | 1 | 2 | 21 | 2 | 1 | 2 | 29 | 7 | |||||
| (Pfu) | – | 4 | 12 | 3 | – | – | 19 | 16 | |||||
| Bat–21 | (T)21 + (TA)11 | 35 | 36 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | |||
| (Taq) | 1 | 1 | 1 | – | 10 | 5 | 8 | 2 | 1 | 29 | 34 | ||
| (Pfu) | – | – | – | 2 | 3 | 1 | 8 | 4 | 1 | 19 | 16 | ||
| Bat–26 | (A)26 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | ||
| (Taq) | – | 1 | 1 | 2 | 1 | 4 | 10 | 12 | 2 | 1 | 34 | 35 | |
| (Pfu) | 1 | 1 | 1 | 5 | 7 | 7 | 8 | 9 | – | 1 | 40 | 23 | |
| D15S128 | (CA)18 | 14 | 15 | 16 | 17 | 18 | |||||||
| (Taq) | – | 1 | 1 | 3 | 9 | 14 | 64 | ||||||
| (Pfu) | 2 | – | 2 | 6 | 5 | 15 | 33 | ||||||
Each locus was amplified by PCR with either Taq or Pfu, as indicated.
The number of clones found containing varying numbers of repeat units is shown, with the correct number of repeats for each locus shown in bold.
N, total number of cloned inserts sequenced for each locus; % Correct, the percentage containing the exact repeat length.