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. 2002 Feb;55(1):29–33. doi: 10.1136/mp.55.1.29

Figure 2.

Figure 2

(A) Electrophoresis using flanking primer pairs produces the following PCR products: lane 1, I/D heterozygote with bands at both approximately 190 and 490 bp; lane 2, II homozygote with a single band of approximately 490 bp; lane 3, DD homozygote with single band at approximately 190 bp. (B) Electrophoresis using insert specific primers produces the following PCR products: lanes 1 and 2 both show single bands representing the insert fragment of 335 bp (I+), lane 3, no bands detected (I−), thus differentiating a true DD homozygote from an I/D heterozygote.