Table 1.

Expression of Wnts, BMPs, and Fz in human tissues and colon cancer cell lines

	Normal colonic mucosa	Colon cancer	Ht29	Colo205	CaCo2	Panc1
Wnt 1	Positive	Positive	Positive	Positive	Positive
Wnt 2	Negative	Hetero positive	Weakly positive	Weakly positive	Positive
Wnt 4	Positive	Positive	Positive	Positive	Positive
Wnt 5A	Positive, stronger at base of crypts	Strongly positive	Positive	Positive	Positive
Wnt 5b	Positive	Hetero positive	Positive	Positive	Positive
Wnt6	Positive	Positive	Positive	Positive	Positive
Wnt 7a	Negative, possible few positive cells at base	Negative	Weakly positive	Weakly positive	Negative
Wnt 7b	Positive	Positive	Positive	Weakly positive	Hetero positive
Wnt 10b	Positive	Positive	Positive	Positive	Positive
BMP 2a	Negative	Negative	Weakly hetero positive	Weakly hetero positive	Weakly hetero positive	Positive
BMP6	Positive	Negative to weakly positive	Positive	Positive	Positive
Fz 1/2	Negative	Positive, poorly differentiated	Positive	Positive	Positive

Panc1 cells were used as a positive control for BMP2a only. Hetero indicates heterogeneous staining among cells visualised in the tissue samples. Wnt and BMP mRNA were detected by in situ hybridisation and Fz1/2 protein by immunoperoxidase. See materials and methods section for numbers of samples analysed.

BMP, bone morphogenetic protein; Fz, frizzled.