Figure 2.

In situ hybridisation for KLK8 mRNA in normal and pathological skin samples. (A) In normal skin, weak hybridisation signals were localised only in the superficial layer shown by arrows. No signals and strong hybridisation signals were found in the lesional skin samples of psoriasis vulgaris using (B) sense and (C) antisense probes for KLK8 mRNA, respectively. Parakeratotic cells localised in the cornified layer did not express KLK8 mRNA (arrowheads). (E) Strong hybridisation signals (asterisk) for KLK8 mRNA were found in lesional skin of seborrheic keratosis. (A, B, C, And E) are bright field micrographs of skin sections counterstained by haematoxylin and eosin; (D) and (F) are dark field micrographs of the same fields shown in (C) and (E), respectively. Scale bars: (A), 50 μm; (B–F), 100 μm.