Figure 5.

Northern blot analysis of KLK8 and tissue type plasminogen activator (tPA) mRNA in cultured human keratinocytes. Total RNA was collected at the stage of 70% confluence (70%) and 100% confluence (100%) in low Ca²⁺ medium (low Ca²⁺). Total RNA was also collected at one day (1D) and three days (3D) of culture after changing to high Ca²⁺ medium (high Ca²⁺). KLK8 mRNA expression increased greatly in parallel with the differentiation of keratinocytes. GAPDH, glyceralaldehyde 3-phosphate dehydrogenase.