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The expression of ADAM12 in six cell cultures from cases of giant cell tumour. The ADAM12 reverse transcription polymerase chain reaction (RT-PCR) product is 498 bp long. Lane 1, 100 bp DNA ladder; lane 2, eighth passage cells; lane 3, 10th passage cells; lane 4, sixth passage cells; lane 5, 12th passage cells; lane 6, fourth passage cells; lane 7, first passage cells. The β actin RT-PCR product from the same culture cells was used as an internal control (lower panel).
