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. 2025 Feb 17;16:1490330. doi: 10.3389/fimmu.2025.1490330

Table 3.

Clinicopathological feathers of AML patients with respect to TSS-A methylation.

Characteristics Hypomethylated TSS-A
(n=117)
Hypermethylated TSS-A
(n=77)
P-value
Median age, year 60 (21-88) 51 (18-76) 0.0002
Median hemoglobin, g/dL 9 (6-14) 9 (6-13) 0.4800
Median leukocytes, ×109/L 15 (1-298) 28 (1-297) 0.3200
Median monocytes, ×109/L 22 (1-94) 14 (0-91) 0.0000
Median platelet 61 (9-351) 36 (8-232) 0.0001
Median BM cellularity, % 90 (10-100) 90 (10-100) 0.0017
Median BM blast, % 17 (0-96) 48 (0-98) 0.0200
Median BM lymphocyte, % 22 (1-94) 14 (0-91) 0.0200
Median BM neutrophil, % 6 (0-94) 6 (0-46) 0.7700
Median TMB, Mut/Mb 0.33 (0-0.90) 0.27 (03-1.13) 0.0200
Gender (male/female) 67/50 38/39 0.3500
FAB 0.0065
 M0 15 (7.73%) 4 (2.06%) 0.0897
 M1 21 (10.82%) 21 (10.82%) 0.1229
 M2 22 (11.34%) 20 (10.31%) 0.2354
 M3 6 (3.09%) 13 (6.70%) 0.0070
 M4 28 (14.43%) 14 (7.22%) 0.3414
 M5 18 (9.28%) 4 (2.06%) 0.0363
 M6 3 (1.55%) 0 (0%) 0.2782
 M7 3 (1.55%) 0 (0%) 0.2782
 Not Classified 1 (0.52%) 1 (0.52%) 1.0000
Cytogenetic abnormalities 0.000
 Normal 58 (32.95%) 42 (23.86%) 0.5594
 t (15;17) 4 (2.27%) 11 (6.25%) 0.0061
 t (8;21) 0 (0%) 7 (3.98%) 0.001
 inv (16) 8 (4.55%) 1 (0.57%) 0.0684
 Trisomy 8 4 (2.27%) 5 (2.84%) 0.3319
 Complex 21 (11.93%) 3 (1.70%) 0.0002
 del (7q)/7q- 6 (3.09%) 1 (0.52%) 0.1549
 del (7q)/7q-|t (9;11) 1 (0.52%) 0 (0%) 0.4118
 del (5q)/5q-|del (7q)/7q- 2 (1.03%) 0 (0%) 0.2444
 del (5q)/5q- 0 (0%) 1 (0.57%) 0.2204
 t (9;11) 2 (1.14%) 0 (0%) 0.2444
 N/A 12 (6.19%) 6 (3.09%)
Cytogenetic-risk categories 0.0075
 Favorable 16 (8.38%) 20 (10.47%) 0.0319
 Intermediate/Normal 66 (34.55%) 47 (24.61%) 0.5402
 Poor 33 (17.28%) 9 (4.71%) 0.0059
 N/A 2 (1.03%) 1 (0.52%)
Gene mutations
 DNMT3A mut (+/-) 40/77 9/68 0.0008
 CEBPA mut (+/-) 2/115 11/66 0.0017
 TP53 mut (+/-) 16/101 0/77 0.0018
 RUNX1 mut (+/-) 14/103 2/75 0.0400
 WT1 mut (+/-) 3/114 8/69 0.0500
 NPM1 mut (+/-) 26/91 27/50 0.0700
 IDH1 mut (+/-) 10/107 9/68 0.6400
 NRAS mut (+/-) 10/107 5/72 0.8000
 FLT3 mut (+/-) 32/85 23/54 0.8300
 TET2 mut (+/-) 9/108 7/70 0.9400

TSS-A, transcription start sites of GCNT2 isoform A; FAB, French–American–British subtypes; BM, bone marrow; TMB, tumor mutational burden; Mut/Mb, mutations per megabase.

Bold values highlight statistically significant differences, bold italic values indicate a trend toward significance.