Immunoblot of tissue lysate from adenoid cystic carcinoma and normal surrounding salivary gland tissue. The 52 kDa WT-1 protein is detected by a polyclonal antibody in tumour tissue lysate but not in normal salivary gland tissue lysate. C, Coomassie staining; M, molecular weight marker (Rainbow High, Amersham, Braunschweig, Germany).