Comparison of methods with spiked samples. (A) Gel of QIAamp method with and without lyticase. (B) Gel of TaKaRa method plus ethanol precipitation, with and without lyticase. (C) Blot of QIAamp methods. (D) Blot of TaKaRa methods. M, molecular weight marker; lanes 1–5, 104, 103, 102, 101, and 100Aspergillus fumigatus conidia, respectively; lane 6, negative control. L, with lyticase.