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. 2024 Oct 22;15(1):526–541. doi: 10.1016/j.apsb.2024.10.006

Figure 2.

Figure 2

In vitro cellular uptake and lysosomal escape. (A) Cell uptake of siGPX4, HFn@Fe/siGPX4 and Antibody + HFn@Fe/siGPX4 in MCF-7 cells for 4 h by CLSM. (B) Uptake of HFn@Fe/siGPX4 in RAW264.7 cells, THP-1 cells and MCF-7 cells for 4 h by CLSM. (C) Cell uptake of siGPX4, HFn@Fe/siGPX4 and Antibody + HFn@Fe/siGPX4 in MCF-7 cells for 4 h by FCM and MFI of Cy5. (D) Uptake of HFn@Fe/siGPX4 in RAW264.7 cells, THP-1 cells and MCF-7 cells for 4 h by FCM and MFI of Cy5. (E) Lysosomal escape of HFn@siGPX4 and HFn@Fe/siGPX4 after incubation with MCF-7 cells for 1 and 4 h by STED. Scale bar = 25 μm. Data are presented as mean ± SD (n = 3). ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.