Skip to main content
. 2024 Oct 22;15(1):526–541. doi: 10.1016/j.apsb.2024.10.006

Figure 3.

Figure 3

In vitro gene silencing effects and cellular ferroptosis induction. (A) The expression level of GPX4 mRNA in MCF-7 cells by RT-qPCR. (B) The expression level of the GPX4 protein in MCF-7 cells by ELISA. (C) The expression level of the GPX4 protein in MCF-7 cells by WB. (D) Intracellular ROS concentration of MCF-7 cells detected by ROS probe DCFH-DA. Scale bar = 100 μm. (E) Quantification of fluorescence intensity based on (D) by ImageJ. (F) Intracellular Fe level of MCF-7 cells. (G) MDA level of MCF-7 cells. (H) Intracellular GSH level of MCF-7 cells. Data are presented as mean ± SD (n = 3). ∗P < 0.05, ∗∗P < 0.01,∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. ns, not significant.