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. 2024 Nov 26;15(1):349–370. doi: 10.1016/j.apsb.2024.11.019

Figure 3.

Figure 3

NEK7 is a new binding partner of PGAM5. (A) Verification of the PGAM5-NEK7 interaction by streptavidin pull-down assay in HEK293T. The Bcl-xL, KEAP1 and DRP1 proteins serve as positive controls for PGAM5-FLAG-APEX2 proximity labeling. (B–D) Immunoprecipitation of the interaction between PGAM5 and NEK7 in the absence or presence of NLRP3 in HEK293T. (E) Immunoprecipitation analysis of the effect of knockdown of PGAM5 on the interaction between PGAM5 and NEK7, similarly between NEK7 and NLRP3 in HEK293T. (F) Immunoprecipitation analysis of the effect of knockdown of NEK7 on the interaction between PGAM5 and NEK7, similarly between PGAM5 and NLRP3 in HEK293T. Similar results were obtained from three independent experiments (A–F).