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. 2025 Feb 19;122(8):e2423853122. doi: 10.1073/pnas.2423853122

Fig. 2.

Fig. 2.

Expression of Endo S2 in Expi293F-GnT1- cells results in cleavage of antibody N-glycans to GlcNAc. (A) The chMC813-70 antibody glycoforms expressed by engineered and parental Expi293F-GNT1- cells were subjected to electrophoresis and a band shifting was observed. (B) The chMC813-70 antibody expressed by engineered Expi293F-GnT1- cells was subjected to intact protein mass analysis to examine the molecular weight and glycan changes. (C) The chMC813-70 antibody expressed by engineered Expi293F-GnT1- cells was subjected to transglycosylation, and the efficiency was evaluated by protein electrophoresis.