Fig. 6.

Improved receptor binding of Fc-SCT-enriched antibody compared to homogeneous and WT antibodies. The Fcγ receptor-coated plate (A), FcγIIA; (B), FcγIIIA; (C), FcγIIB; (D), FcγIIIB was incubated with humanized WT antibody (black), homogeneous SCT antibody (blue), or Fc-SCT-enriched antibody (red) at indicated concentrations. The binding of the antibody was measured by anti-human IgG Fc antibody conjugated with HRP. The binding of Fc-SCT-enriched antibody to receptors was greatly improved compared with WT and homogeneous Fc-SCT-humanized antibodies.