. 2025 Feb 8;20:100985. doi: 10.1016/j.onehlt.2025.100985

Table 2.

Primers used in the qPCR for the detection of the free-living amoebae (FLA) in this study.

FLA species	Primers sequence	DNA fragment (bp)
Acanthamoeba spp.^⁎	AcantF900 (5`-CCC AGA TCG TTT ACC GTG AA-3`) AcantR1100 (5`-TAA ATA TTA ATG CCC CCA ACT ATC C-3`) AcantProb (5`-JUN-CT GCC ACC GAA TAC ATT AGC ATG G-QSY-3`)	180
Naegleria fowleri^⁎	NaeglF192 (3`-GTG CTG AAA CCT AGC TAT TGT AAC TCA GT-5`) NaeglR344 (5`-CAC TAG AAA AAG CAA ACC TGA AAG G-3`) NeglProb (5`-VIC-AT AGC AAT ATA TTC AGG GGA GCT GGG C-QSY-3`)	153
Balamuthia mandrillaris^⁎	BalaF1451 (5`-TAA CCT GCT AAA TAG TCA TGC CAA T-3`) BalaR1621 (5`-CAA ACT TCC CTC GGC TAA TCA-3`) BalaProb (5`-6FAM-AG TAC TTC TAC CAA TCC AAC CGC CA-QSY-3`)	171
Vermamoeba vermiformis	Hv1227F (5′-TTA CGA GGT CAG GAC ACT GT- 3′)^⁎⁎ VermRv (5´ TGCCTCAAACTTCCATTCGC 3′)^⁎⁎⁎ VermProb (5`-ABI-TTG ATT CAG TGG GTG GTG GT-QSY-3`)^⁎⁎⁎	235

^⁎

Primers sequence described in [40]

^⁎⁎

Forward primer sequence adapted from a previous study and described in [9].

^⁎⁎⁎

This reverse primer sequence was specially designed in [9].