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. 2025 Jan 21;21(3):231–253. doi: 10.1038/s44320-025-00085-6

Reagents and tools table

Reagent/Resource Reference or Source Identifier or Catalog Number
Experimental models
AsPC-1 (H. sapiens) ATCC CRL-1682
Additional pancreatic cancer cell lines See Appendix Table S1 See Appendix Table S1
Pancreatic patient-derived organoids See Appendix Table S2 See Appendix Table S2
Chemicals, Enzymes and other reagents
Elimusertib Selleckchem S8666
Gartisertib Selleckchem S9639
Ceralasertib Selleckchem S7693
Berzosertib Selleckchem S7102
Gemcitabine Selleckchem S1714
Oxaliplatin Selleckchem S1224
Cisplatin Selleckchem S1166
5-Fluorouracil Selleckchem S1209
Additional compounds used in viability screen See Dataset EV1 See Dataset EV1
TMT10plex Isobaric Label Reagent Set Thermo Fisher Scientific 90406
TMT11-131C Labeling Reagent Thermo Fisher Scientific A34807
TMTpro 16plex Label Reagent Set Thermo Fisher Scientific A44520
DMEM PAN Biotech P04-04510
RPMI PAN Biotech P04-18500
DMEM/Hams-F12 (1:1) PAN Biotech P04-41154
Fetal Bovine Serum PAN Biotech P04-18500
HEPES PAN Biotech P05-01100
Non Essential Amino Acid Solution PAN Biotech P08-32100
Bovine Pituitary Extract Thermo Fisher Scientific 13028014
Recombinant human EGF protein R&D Systems 236-EG
Keratinocyte-free medium Thermo Fisher Scientific 17005042
Penicillin Streptomycin GlutaMAX Thermo Fisher Scientific A5873601
Reagents used for patient-derived organoids See Appendix Table S3 See Appendix Table S3
ATPlite 1step Luminescence Assay System revvity, previously Perkin Elmer 6016731
CellTiter-Glo Luminescent Cell Viability Assay Promega G7572
Software
MaxQuant (v1.6.12.0, v2.1.3.0) Cox and Mann (2008)
Perseus (v1.6.2.3) Tyanova et al (2016)
SIMSI-Transfer (v0.5.0) Hamood et al (2022), https://github.com/kusterlab/SIMSI-Transfer
CurveCurator (v0.4.1) Bayer et al (2023), https://github.com/kusterlab/curve_curator
PTMnavigator Müller et al (2025), https://www.proteomicsdb.org/analytics/ptmNavigator
Other
Dionex UltiMate 3000 RSLCnano System Thermo Fisher Scientific
Fusion Lumos Tribrid mass spectrometer Thermo Fisher Scientific
Janus Gripper revvity, previously Perkin Elmer
Multidrop Combi Reagent Dispenser Thermo Scientific
Incucyte S3 Sartorius
Envision Xcite 2104 plate reader revvity, previously Perkin Elmer
Spark Multimode microplate reader TECAN
VantaSTAR BMG Labtech