Reagents and tools table
| Reagent/resource | Reference or source | Identifier or catalog number |
|---|---|---|
| Experimental models | ||
| S. cerevisiae cell lines | This study | Appendix Table S1 |
| Rosetta2(DE3) E. coli | Sigma Aldrich | 71397-3 |
| Recombinant DNA | ||
| Plasmids for yeast strain generation and recombinant protein expression. | This study | Appendix Table S2 |
| Antibodies | ||
| α-Cse4 | Pinsky et al, 2003 | 9536 |
| α-Ndc10 | Lang et al, 2018 | OD1 |
| α-Ame1 | This study, Genscript | 2181 |
| α-V5 | Invitrogen | R96025 |
| α-Rabbit IgG peroxidase-linked species-specific whole antibody (from donkey) Secondary Antibody | Cytiva | NA9341ML |
| Oligonucleotides and other sequence-based reagents | ||
| Oligonucleotides | This study | Appendix Table S3 |
| Chemicals, enzymes, and other reagents | ||
| Protocatechuic acid | Sigma Aldrich | PHL89766 |
| Protocatechuate 3,4-Dioxygenase from Pseudomonas sp. | Sigma Aldrich | P8279 |
| 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid | Sigma Aldrich | 238813 |
| BIO-PEG-SVA, MW 5,000 and MPEG-SVA, MW 5,000 | Laysan Bio | BIO-PEG-SVA-5K-100MG & MPEG-SVA-5K-1g |
| Avidin DN | Vector Laboratories | A-3100-1 |
| Vectabond Tissue Section Adhesive | Vector Laboratories | SP-1800 |
| Phusion High-Fidelity DNA Polymerase | New England Biolabs | M0530S |
| SuperSignal™ West Dura Extended Duration Substrate | Thermo Fisher | 34075 |
| Software | ||
| Fiji | https://fiji.sc | |
| MATLAB | https://matlab.mathworks.com | |
| ASMCA |
Popchock et al, 2023 https://github.com/FredHutch/Automated-Single-Molecule-Colocalization-Analysis |
|
| Other | ||
| Nikon TE-2000 inverted RING-TIRF microscope | Nikon | |
| DeltaVision Ultra | GE Healthcare | |
| ChemiDoc Imager | Bio-Rad | |