Indicate in the comments box below whether you are happy with the changes made or if the manuscript is unacceptable.

Comments on revised manuscript

Q1：Why is the estimated genome size from the genome survey much smaller than the assembled genome size? Q2:In the method section, I did not see a description of the de novo method for gene structure annotation. Q3:I am concerned about using a reference genome with unclear karyotype relationships for scaffolding. Q4:Are there other published comparative genomic studies on deer that have identified such a small number of homologous genes?