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Subclones of p66 expressed as Gal4 and EGFP fusions used for interaction analysis. The regions of p66 shown were expressed in mammalian and two hybrid vectors and were made as described in the Methods section. The constructs p66Δ11, p66Δ21 and p66Δ31 have the C-terminal 11, 21 and 31 amino acids deleted, respectively. p66S2 expresses amino acids 1–144; p66S1 expresses amino acids 1–375.
