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. 2005 Aug 18;115(9):2393–2401. doi: 10.1172/JCI25437

Figure 8.

Figure 8

Confirmation of a selective blockade of TRPA1 expression in a time-dependent manner. (A and B) TRPA1 AS-ODN (0.5 nmol/μl–1/h–1) attenuates the induction of TRPA1, but not TRPM8, mRNA induced by peripheral inflammation and injury to the L5 spinal nerve. Quantification of the percentage of TRPA1 (A) and TRPM8 (B) mRNA–positive neurons at day 3 after CFA injection or in the L4 DRG at day 7 after L5 SNL. Data represent mean ± SD; n = 4 per group. (C) Fluorescence in the naive DRG and the DRG at 6 and 24 hours after intrathecal injection of FITC-labeled ODN. Scale bar: 50 μm. (D) Effect of treatment with TRPA1 AS-ODN (0.5 nmol/μl–1/h–1) following CFA injection on CFA-induced cold hyperalgesia at days 1 and 3. Data represent mean ± SEM; n = 8 per group. *P < 0.05 compared with naive control. #P < 0.05 compared with MM-ODN (0.5 nmol/μl–1/h–1) group.