Localisation of Dam1 is dependent on Spc34, Ask1, Duo1 and Dad1. (A) Wild-type, Δspc34, Δduo1 and Δask1 cells were streaked either onto rich medium at 30°C containing either no addition (control), 10 μg/ml TBZ, 0.6 M KCl or on rich medium at 37°C and incubated for 3 days. (B) Log phase dam1-gfp cdc11-cfp, Δduo1 dam1-gfp cdc11-cfp, Δspc34 dam1-gfp cdc11-cfp, Δask1 dam1-gfp cdc11-cfp and Δdad1 dam1-gfp cdc11-cfp cells were fixed and stained with DAPI and calcofluor and visualised by fluorescence microscopy. Scale bar=5 μm.