Figure 9.

Dam1 and Klp5 have distinct effects on sister chromatid separation in rapidly dividing cells. (A) Log phase cen1-gfp cdc11-cfp:kanR cells (open squares), Δdam1 cen1-gfp cdc11-cfp:kanR cells (open circles) or Δklp5 cen1-gfp cdc11-cfp:kanR cells (closed squares) were synchronised in early G2 and incubated in fresh medium and the percentage of binucleate cells was expressed as a percentage of the total number of cells at the indicated time points (n=300). (B) The cells from (A) were fixed after 90 min and scored for either segregation of sister chromatids to opposite spindle poles (normal segregation), segregation of only one of the two sister chromatids to the spindle pole (lagging sister chromatid) or segregation of both sister chromatids to the same pole (unequal segregation). Scale bar=5 μm. The percentage of each segregation event was expressed as a percentage of the total number of cells (n=250).