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. 2005 Aug 17;33(15):4673–4682. doi: 10.1093/nar/gki774

Figure 6.

Figure 6

Transcript analysis of C24 × Col and Col × C24 hybrids. (A) Northern hybridization of total RNA from ecotypes C24, Col, Ler (lanes 1–3) and each two plants (A and B) of C24 × Col and Col × C24 hybrids (lanes 4–7). The C24 specific RNAs of ∼1560 and 2800 nt are detected in the C24 parental line (lane 1) and in hybrids with C24 female parents (lanes 4 and 5). (B) Primer extension analysis of the same RNAs as described in (A) with primer Atcox3-3. Products of ∼160 nt (1) were generated on RNAs of C24 (lane 1) and of plants A and B with C24 as female parental line (lanes 4 and 5). These products correspond to 5′ ends at positions around −440, which are exclusively detected in the CR–RT–PCR of this ecotype (Figure 5A and D). A second prominent product (2) of ∼100 nt is detected in almost all plants, except in C24 in lane 1. This ends corresponds to 5′ termini around −380, which was found in the CR–RT–PCR in all ecotypes (Figure 5).