Fig. 1.

Structure of the cnk-1 gene and domains of the CNK-1 protein. (A) cnk-1 genomic structure and location of deletion mutations. The exons are depicted as boxes and the introns as connecting lines. The black boxes depict regions encoding the sterile alpha motif (SAM), conserved region in Cnk (CRIC), PSD-90/Dlg/ZO-1 (PDZ), and Pleckstrin homology (PH) protein domains. The sv39 and ok836 deletions are represented as a line below the genomic structure (see Materials and Methods). (B) Schematic representation of CNK-1, Drosophila CNK, and human CNK2A proteins. RIR, Raf inhibitory region; CRAC, conserved region among chordates. CNK2A is the most closely related of the three mammalian CNK proteins to CNK-1 and also the only one so far implicated in Ras/Raf signaling (24, 26).