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. 2005 Aug 9;102(33):11894–11899. doi: 10.1073/pnas.0501434102

Fig. 2.

Fig. 2.

Conservation of DNA sequence in human and rat GABRA4 promoters. (A) Alignment and identification of potential regulatory elements was performed by using GENOMATIX software. Only sites from matrix families shared by both the human and rat promoter, irrespective of position, are included. Core similarity of 0.75 and matrix similarity of 1.01 were used for detection. Colored bars indicate consensus sites specific to the factors abbreviated as follows on the right: AHR/ARNT, Aryl hydrocarbon receptor; EGR3, Egr gene 3 product; EGR1, Egr-1/Krox-24/NGFI-A/zif268 immediate-early gene product; WT1, Wilms tumor suppressor; HES1, Drosophila hairy and enhancer of split homologue 1; MAZ, Myc-associated zinc-finger protein; MTF, metal transcription factor 1; NF1, nuclear factor 1; P53, tumor suppressor p53; SP1, stimulating protein 1; RFX1, X-box binding protein R; MIF1, MIBP-1-RFX1 complex; ZF5, zinc finger/poxvirus zinc finger (POZ) domain transcription factor. Detection of major transcription initiation site for human and rat GABRA4 was performed by using RACE with either rat RNA from cultured HNs (most-5′ site indicated by line corner and plus) or human brain RNA (down-stream site indicated by line corner and plus). The 5′ end of the adult mouse hippocampal EST obtained from a search of the National Center for Biotechnology Information database (accession no. BY712705) is indicated by a black rectangle, and circles indicate the 5′ ends of adult human hippocampal ESTs from the National Center for Biotechnology Information database (accession nos. BI545886 and BG699585). Conserved sequence is indicated by stars, and shared sequences between elements are indicated by overlapping bars. The numbering of sequence is in reference to the first nucleotide (+1) of the human GABRA4 gene as determined by RACE. (B) Start-site identification using cDNAs generated from either rat primary hippocampal cultures or human brain total RNA. (Left) Lanes are defined as follows: M, 50 bp DNA ladder marker; -,no template; Control, control mouse thymus cDNAs as template; and Rat, rat hippocampal cDNAs as template. (Right) Lanes are defined as follows: M, 50 bp marker; and Human, human brain cDNAs as template.