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Ser-/Ala-tRNAAla deacylation by AlaX/AlaRS. Shown are the deacylation of Ser-tRNAAla (A) and Ala-tRNAAla (B) by wild-type PhoAlaXM (WT-AlaXM), wild-type PhoAlaXD (WT-AlaXD), PhoAlaXM-T30V mutant (AlaXM-T30V), wild-type PhoAlaRS (WT-AlaRS), and PhoAlaRS-Q633M mutant (AlaRS-Q633M), or without the enzymes (no enzyme) at 55°C.
