Table 2.
MIC values and genetic features associated to cefiderocol susceptibility/resistance among P. aeruginosa clinical isolates
| Strain | Sample origin and date (MM/YY) | FDCa MIC value (mg/L) |
β-Lactamase contentb | ST-type | TonB-dependent receptor proteinsc | Iron uptake system proteinsc | Porin proteinc |
Efflux regulators proteinsc | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| piuA/piuD | piuB | pirA | pirR | pirS | fecA | fecI | oprD | mexR | nalD | |||||
| PA36 |
Bern 01.2022 |
16 | OXA-851 (c), PDC-322 (c) | 645 | Q34H |
A573T H604N |
A370T | Truncated |
Q77R N126S G360D |
V95A H363R |
WT | disrupted | WT | Q213_P265Ins |
| PA48 |
Zürich 01.2022 |
4 | PDC-35 (c), GES-7 (c), OXA-488 (c) | 235 | T411I* | A609V |
A370T S20N T235I |
WT | WT |
V95A A113V T288I T339A G358S T359A H363R R571Q |
WT | disrupted | V126E | V151_M158del |
| PA82 |
Luzern 01.2022 |
4 | OXA-488 (c), PDC-34 (c) | 253 | Q34H | WT | A370T | WT | WT |
V95A S2F T339A G358S T359A H363R R571Q |
WT | disrupted | V126E | Truncated |
| PA54 |
Luzern 03.2022 |
4 | PDC-35 (c), GES-7 (c), OXA-488 (c) | 235 | T411I* | A609V |
A370T S20N T235I |
WT | WT |
V95A A113V T288I T339A G358S T359A H363R R571Q |
WT | disrupted | V126E | V151_M158del |
| PA76 |
Geneva 03.2022 |
4 | OXA-395, PDC-16, NDM-1 (p) | 773 | Q34H |
G382S A609V |
A370T | WT | N126S |
V95A T298A T339A G358S T359A H363R R571Q |
WT | disrupted | V126E | WT |
| PA08 |
Bern 05.2022 |
64 | OXA-1032 (c), PDC-22 (c) | 667 |
Q34H Truncated |
NA | A370T |
F2L E69D |
WT |
V95A V212A G358S T359A T339A H363R R571Q |
WT | disrupted | Truncated | WT |
| PA72 |
Luzern 07.2022 |
4 | OXA-395 (c), PDC-16 (c), NDM-1 (p) | 773 | Q34H |
G382S A609V |
A370T | WT | N126S |
V95A T298A T339A G358S T359A H363R R571Q |
WT | disrupted | V126E | WT |
| PA10 |
Bern 07.2022 |
4 | OXA-395 (c), PDC-16 (c), NDM-1 (p) | 773 | Q34H |
G382S A609V |
A370T | WT | N126S |
V95A T298A T339A G358S T359A H363R R571Q |
WT | disrupted | V126E | WT |
| PA136 |
Zürich 07.2022 |
4 | PDC-3 (c), OXA-395 (c), VIM-2 (p) | 111 | WT * |
P51S A143V A573T H604N |
A370T | A52G |
Q77R N126S L213F G360D |
A342V H363R |
ND | disrupted | V126E | WT |
| PA58 |
Basel Land 08.2022 |
4 | OXA-395 (c), PDC-30 (c) | 207 | Q34H |
F165L D574N A594V H604N |
A370T K590T D608G |
F2L | N126S |
V95A T339A G358S T359A H363R R571Q |
WT | disrupted | V126E | R13C |
| PA12 |
Zürich 09.2022 |
4 | VIM-5 (c), OXA-846 (c), PDC-11 (c), VEB-14 (p), OXA-10 (p) | 357 | WT * |
F165L L197F A351V A609V |
Y2S A370T |
WT | A304V |
V95A T339A G358S T359A H363R R571Q |
WT | disrupted | V126E | Truncated |
| PA69 |
Tessin 11.2022 |
4 | OXA-395 (c), PDC-16 (c), NDM-1 (p) | 773 | Q34H |
G382S A609V |
A370T | WT | N126S |
V95A T298A T339A G358S T359A H363R R571Q |
WT | disrupted | V126E | WT |
| PA72 |
Luzern 11.2022 |
4 | OXA-395 (c), PDC-16 (c), NDM-1 (p) | 773 | Q34H |
G382S A609V |
A370T | WT | N126S |
V95A T298A T339A G358S T359A H363R R571Q |
WT | disrupted | V126E | WT |
| PA13 |
Luzern 12.2022 |
8 | PDC-16 (c), OXA-395 (c), NDM-1 (p) | 773 | Q34H |
G382S A609V |
A370T | WT | N126S |
V95A T298A T339A G358S T359A H363R R571Q |
WT | disrupted | V126E | WT |
| PA35 |
Zürich 12.2022 |
4 | PDC-16 (c), OXA-395 (c), NDM-1 (p) | 773 | Q34H |
G382S A609V |
A370T | WT | N126S |
V95A T298A T339A G358S T359A H363R R571Q |
WT | disrupted | V126E | WT |
| PA53 |
Basel Land 12.2022 |
4 | PDC-16 (c), OXA-395 (c), NDM-1 (p) | 773 | Q34H |
G382S A609V |
A370T | WT | N126S |
V95A T298A T339A G358S T359A H363R R571Q |
WT | disrupted | V126E | WT |
| PA23 |
Zürich 05.2022 |
0.25 | OXA-395 (c), PDC-3 (c), OXA-9 (p), VIM-4 (p) | 111 | WT |
P51S A143V A573T H604N |
A370T | A52G |
Q77R N126S V180I L213F G360D |
A342V | NA | disrupted | V126E | WT |
| PA24 |
Zürich 05.2022 |
0.25 | PDC-35 (c), OXA-488 (c), VIM-2 (p) | 235 | T411I* | A609V |
S20N T235I A370T |
WT | WT |
V95A A113V T288I T339A G358S T359A R571Q |
WT |
T103S K115T F170L P186G V189T R310E A315G G425A |
V126E | WT |
| PA65 |
Bellinzona 06.2022 |
0.25 | OXA-488 (c), PDC-30 (c), VIM-2 (p) | 671 | K729Q* |
S454C Q465H A573T |
V128A A370T |
WT |
Q77R N126S |
V95A A298V T339A G358S T359A R571Q A714V |
WT |
S57E S59R V127L P186G V189T I210A E230K S240T N262T T276A K296Q Q301E R310E A315G L347M V372_G383Ins S403A Q426E |
WT | WT |
| PA70 |
Zürich 07.2022 |
0.25 | PDC-35 (c), OXA-488 (c), VIM-5 (p) | 235 | T411I* | A609V |
S20N T235I A370T |
WT | WT |
V95A A113V T288I T339A G358S T359A R571Q |
WT |
T103S K115T F170L P186G V189T R310E A315G G425A |
V126E | WT |
| PA90 |
Buchs 07.2022 |
0.25 | OXA-905 (c), PDC-8 (c), OXA-10 (p), VIM-2 (p) | 395 | WT* |
S311N A384V D574N A594V H604N E806G |
A370T | A52G |
Q77R N126S V180I E272D Q274R D328A |
WT | WT |
D43N S57E S59R I210A E230K S240T N262T A267S K296Q Q301E R310G V359L V372_G383Ins |
WT | WT |
| PA16 |
Luzern 07.2022 |
0.5 | PDC-12 (c), OXA-488 (c), OXA-10 (c), IMP-1 (c) | 1047 | Q34H | E785G | A370T | WT | WT |
V95A G358S T359A R571Q |
WT |
T103S K115T F170L P186G V189T R310E A315G G425A |
V126E | NA |
| PA38 |
Zürich 07.2022 |
0.25 | PDC-35 (c), OXA-488 (c), VIM-2 (c), NDM-1 (c) | 235 | T411I* | A609V |
S20N T235I A370T |
WT | WT |
V95A A113V T288I T339A G358S T359A R571Q |
WT | disrupted | V126E | WT |
| PA89 |
Sion 08.2022 |
0.25 | OXA-395 (c), PDC-3 (c), OXA-9 (p), VIM-4 (p) | 111 | WT* |
P51S A143V A573T H604N |
A370T | A52G |
Q77R N126S V180I L213F G360D |
A342V | NA | disrupted | V126E | WT |
| PA03 |
Liebefeld 08.2022 |
0.25 | OXA-395 (c), PDC-3 (c), VIM-2 (p) | 111 | WT* |
P51S A143V A573T H604N |
A370T | A52G |
Q77R N126S V180I L213F G360D |
A342V | NA | disrupted | V126E | WT |
| PA18 |
Bellinzona 08.2022 |
0.5 | PDC-19a (c), OXA-488 (c), NDM-1 (p) | 308 |
Q34H P79L |
F165L G844R |
A370T | WT |
N126S S260P |
V95A Q122P T339A G358S T359A R571Q A714V |
WT |
T103S K115T F170L P186G V189T R310E A315G G425A |
V126E | Truncated |
| PA40 |
Geneva 08.2022 |
0.5 | PDC-3 (c), OXA-395 (c), VIM-2 (p) | 111 | Q34H |
P51S A143V A573T H604N |
A370T | A52G |
Q77R N126S V180I L213F G360D |
A342V | NA | disrupted | V126E | WT |
| PA29 |
Bern 09.2022 |
2 | OXA-488 (c), PDC-46 (c) | 1917 | Q34H |
F165L D574N A594V H604N |
A370T K590T D608G |
Truncated F2L |
N126S V180I S260P |
V95A F155L T339A G358S T359A R571Q |
WT | disrupted | V126E | WT |
| PA98 |
Bern 07.2022 |
2 | OXA-396 (c), PDC-3 (c), GES-1 (c), NDM-1 (c) | 654 | WT* |
T301I A573T E598D H604N |
P275S A370T G578E |
A52G |
Q77R N126S V180I D328A |
A27T V95A A120T |
WT |
V127L P186G V189T I210A E230K S240T N262T T276A K296Q Q301E R310E G312R A315G G316D L347M V372_G383Ins S403A Q426E |
WT | L153Q |
| PA47 |
Bern 10.2022 |
0.5 | OXA-488 (c), PDC-158 (c), VIM-2 (p) | 2644 | K729Q* | WT |
A370T R549S T683I |
WT |
N126S S260P |
V95A T339A G358S T359A R571Q |
G13D | disrupted | V126E | WT |
| PA57 |
Zürich 10.2022 |
0.25 | OXA-10 (c), OXA-488 (c), PDC-12 (c), IMP-1 (c) | 1047 | Q34H | E785G | A370T | WT | WT |
V95A G358S T359A R571Q |
WT |
T103S K115T F170L P186G V189T R310E A315G G425A |
V126E | NA |
| PA176 |
Sion 11.2022 |
0.25 | OXA-396 (c), PDC-3 (c), GES-5 (c) | 654 | WT* |
T301I A573T E598D H604N |
P275S A370T G578E |
A52G |
Q77R N126S V180I D328A |
A27T V95A A120T |
WT |
V127L P186G V189T I210A E230K S240T N262T T276A K296Q Q301E R310E G312R G314D A315G L347M V372_G383Ins S403A Q426E |
WT | WT |
(a) FDC. Cefiderocol (b) (c), chromosomally-encoded ß-lactamase; (p), plasmid-mediated ß-lactamase; * substitution found in the piuD gene, (c) WT, wild-type gene; NA. data not available