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Quantification of CX3CR1 mRNA expressing cells in defined lesional stages. (a) Density of silver grains (defined as areas exceeding the mean background + 11 SD) per square unit. (b) Number of CX3CR1 mRNA-expressing cells positively stained with GSI-B4 isolectin. (c) Correlation between total number of GSI-B4 cells and intensity of in situ hybridization signal (density of silver grains per square unit).
