Fig. 2.

Genetic selection and differential expression analysis identify important genes. (A) Manhattan plot of Z-Fst for the RFI population, highlighting the top 5 % as significant regions. (B) Manhattan plot of ROH islands for high and low RFI lines, highlighting the top 1 % as significant regions. (C) PCA of samples from high (red) and low (green) RFI lines. (D) Volcano plot of DEGs between high and low RFI lines. Red indicates up-regulated genes, green indicates down-regulated genes. (E) Expression of PSGs in the RFI population (LRFI/HRFI). Asterisks indicate P value < 0.05, and genes in red are both PSGs and DEGs. (F) Enrichment analysis for upregulated and downregulated DEGs between high and low RFI lines.