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. 2025 Feb 6;104(3):104885. doi: 10.1016/j.psj.2025.104885

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© 2025 The Authors. Published by Elsevier Inc. on behalf of Poultry Science Association Inc.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig 3 — Differences in liver metabolites between high and low RFI lines. (A) OPLS-DA score plot of metabolites from high and low RFI line samples. (B) OPLS-DA S-plot. Red points indicate metabolites with VIP > 1, green points denote VIP ≤ 1. (C) Classification and quantity of differential metabolites. (D) Variations in bile acid content between high and low RFI lines (LRFI/HRFI). (E) Levels of free cholesterol (FC), total bile acid (TBA), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL) by lipid biochemical analysis. The values are the mean ± SEM, * represents P < 0.05, ** represents P < 0.01 and *** represents P < 0.001. (F) Differential gene expression related to cholesterol transport, steroid biosynthesis and apolipoproteins (LRFI/HRFI). (G) Schematic representation of lipid content and gene expression changes in cholesterol metabolism pathways in the liver of the LRFI line.