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. 2025 Feb 6;104(3):104885. doi: 10.1016/j.psj.2025.104885

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© 2025 The Authors. Published by Elsevier Inc. on behalf of Poultry Science Association Inc.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig 4 — Differences in liver lipid profiles across RFI divergent chickens. (A) Lipid class and quantity detected by lipidomic analysis. (B) OPLS-DA score plot of metabolites in high and low RFI samples. (C) Free fatty acid (FFA) and triglyceride (TG) contents in lipidomic and lipid biochemical analysis. (D) Differential content of FFA between high and low RFI groups (LRFI/HRFI). (E) Differential expression of key genes in lipid metabolism pathways (LRFI/HRFI). (F) GSSG levels in high and low RFI chicken livers. (G) MDA levels in high and low RFI chicken livers. (H) 4-HNE levels in high and low RFI chicken livers. (I) Differential expression of oxidative stress-related genes (LRFI/HRFI). (J) Schematic representation of changes in lipid content and gene expression in fatty acid metabolism pathways in the LRFI chicken livers. The values are the mean ± SEM, * represents P < 0.05, ** represents P < 0.01 and *** represents P < 0.001.