TABLE 2.
Escherichia coli strains used in this study.
| Strain | Genotype | Reference/Origin |
|---|---|---|
| NEB® 5-alpha | E. coli fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80Δ (lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | NEB™ |
| BL21 (DE3) | E. coli fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS | NEB™ |
| MG1655 | F − λ - ilvG- rfb-50 rph-1 | ATCC |
| bWL1221 | E. coli MG1655 lldD proD-kan | INSA Toulouse |
| EcHS0 | E. coli MG1655 ΔthrB ΔmetA ΔldhA | This study |
| EcHS1 | EcHS0 with pZA23-HS1-5Q (Ec.aspC, Ec.mdh 5Q ) | This study |
| EcHS2 | EcHS0 with pZA23-HS1-7Q (Ec.aspC, Ec.mdh 7Q ) | This study |
| EcHS3 | EcHS0 with pZA23-HS2-5Q (Ec.alaC A142P:Y275D , Ec.mdh 5Q ) | This study |
| EcHS4 | EcHS0 with pZA23-HS2-7Q (Ec.alaC A142P:Y275D , Ec.mdh 7Q ) | This study |
| EcHS5 | EcHS3 with ΔpfkA | This study |
| EcHS6 | EcHS3 with ΔsthA | This study |
| EcHS7 | EcHS3 with pntAB proD | This study |
| EcHS8 | EcHS7 with ΔsthA | This study |
| EcHS9 | EcHS4 with ΔpfkA | This study |
| EcHS10 | EcHS4 with ΔsthA | This study |
| EcHS11 | EcHS4 with pntAB proD | This study |
| EcHS12 | EcHS11 with ΔsthA | This study |