Fig. 1.

Amplification of the PKCι gene and increased PKCι RNA expression in ovarian cancer. (a) Array comparative genomic hybridization analysis of PKCι DNA copy number in 235 Grade 3 and Stage III or IV serous epithelial ovarian cancer samples (log₂ ratio of cancer patient DNA to normal DNA). (b) Increase in PKCι DNA copy number is associated with a decreased progression-free survival period. For patients where followup information was available, progression-free survival in patients with high PKCι DNA copy number (n = 26) was significantly worse (P = 0.0006) than in patients with low PKCι copy number (n = 46) (cutoff at 0.37 log₂). Vertical lines indicate censored patients, i.e., patients for whom no further followup information was available after the indicated time points. (c) Microarray analysis of PKCι gene expression. Two different studies using Affymetrix DNA microarray analysis (17, 18) show marked elevation of PKCι gene expression in serous epithelial ovarian cancers as compared with pooled (I) and normal ovarian (II) epithelium.