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. 2005 Aug 9;390(Pt 1):19–27. doi: 10.1042/BJ20050324

Figure 3. Multiple sequence alignment of selected fragments of GH68 family members.

Figure 3

The sequences are divided into two groups, Gram-negative and Gram-positive levansucrases headed by LsdA (Gd_lvs) and Bs levansucrase (Bs_lvs) respectively. Protein identifiers and access codes for each sequence in the SwissProt/TrEMBL and GenBank® databases are as follows: Gd LsdA (Gd_lvs, Q43998); B. pseudomallei K96243 levansucrase (SACB_Burps, YP_110564); B. cepacia R1808 hypothetical protein (hyp_Burce, ZP_00218900); Z. mobilis levansucrase (SACB_ZYMMO, Q60114, Q06487, Q60116), and invertase (invB_ZYMMO, AAA61488); Gluconacetobacter xylinus levansucrase (LsxA_Gxyl, BAA93720); Ps. syringae pv. tomato levansucrase (SACB_Psyr1, AAO54974; SACB_Psyr2, AAO55819; SACB_Psyr3, AAO59056); Ps. syringae pv. glycinea levansucrase (SACB_PSESG, O52408; SACB_PsyrG2, AAK49952); Ps. syringae pv. phaseolicola levansucrase (SACB_PSESH, O68609); R. aquatilis levansucrase (SACB_RAHAQ, O54435); Erwinia amylovora levansucrase (SACB_ERWAM, Q46654); Ps. aurantiaca levansucrase (SACB_Paura, AAL09386); Bs levansucrase (Bs_lvs, P05655, P70984); Bacillus stearothermophilus levansucrase (SACB_BACST, P94468); Bacillus amyloliquefaciens levansucrase (SACB_BACAM, P21130); Bacillus licheniformis unnamed protein product (unk_Blich, CAF05486); Clostridium acetobutylicum levansucrase (SACB_Cace, CAC1772; SACB_Cace1, CAC1774); Bacillus sp. V230 β-fructofuranosidase (bff_BspV230, BAA32083); Pa. polymyxa levansucrase (SACB_Ppol, CAB39327); Streptococcus salivarius levansucrase (SACB_STRSL, Q55242); L. reuteri inulosucrase (inu_Lreu, AAN05575), levansucrase (SACB_Lreu, AAO14618) and unnamed protein product (unk_Lreu, CAD19335); Lactobacillus johnsonii levansucrase (SACB_Ljohn, NP_964768); Streptococcus mutans levansucrase (SACB_STRMU, P11701); Lactobacillus sanfranciscensis levansucrase (SACB_Lsanf, CAD48195); Arthrobacter sp. K-1 β-fructofuranosidase (bff_AspK1, BAB72022); Ac. naeslundii FTF (ftf_Anae, AAG09737). Secondary-structural elements were assigned using DSSP [48] and are indicated by ‘e’ (β-strands) and ‘h’ (helices). Catalytic residues Asp135, Asp309 and Glu401 are boxed.