Figure 2. Effects of S14 overexpression on the growth properties of MCF-7 cells.

(A) MB and MS14 cells were grown as described in the Experimental section and the incorporation of [³H]thymidine was determined. Results are the means for three determinations from three different experiments. ***P<0.001. (B) MB and MS14 cells were grown as described in the Experimental section and immunostained with an anti-BrdUrd antibody. The images were obtained using a laser-scanning confocal microscope. (C) Representative colony-forming assay in MB and MS14 cells. Experiments were repeated three times on duplicate samples.