Figure 4. Pharmacological properties of AT₁ receptor glycosylation mutants.

Broken COS-7 cells expressing the AT₁-WT receptor and various glycosylation mutants were incubated for 1 h with increasing concentrations of ¹²⁵I-[Sar¹,Ile⁸]AngII. Non-specific binding was assessed in the presence of 1 μM unlabelled [Sar¹,Ile⁸]AngII. Affinity (K_d) and B_max values were evaluated from the saturation curve data by Scatchard analysis, which is shown to emphasize the decrease in B_max (A) and the decrease in both B_max and K_d of the ECL1 mutants (B). Each data point represents the mean of triplicate values. These results are representative of at least three independent experiments.