Fig. 4. Segmentation results on the H&E Cryo data.
a Model prediction based segmentation masks (“Segmentation”, “Shape Filter” and “Connective Tissue”) for an exemplary selected H&E Cryo whole slide image (WSI) of normal soleus/gastrocnemius skeletal muscle tissue derived from a wild-type mouse (WT). b To assess the model performance of U-Netreal and U-Netsynth on the unlabeled H&E Cryo dataset, the three segmentation masks were rated by two experts (P1 and P2) according to an ordinal scale. The top row exemplary shows two H&E Cryo expert pre-defined ROIs with the respective qualitative segmentation results of U-Netreal and U-Netsynth. The ROIs refer to normal and myopathic soleus/gastrocnemius skeletal muscle tissue derived from wild-type siblings (WT) and homozygous desmin knock-out mice (DKO Hom), respectively. The expert ratings for the ROIs are denoted above the predictions for each category (“Segmentation”, “Shape Filter” and “Connective Tissue”), respectively.