Table 2.

Utilised oligonucleotides

Utilisation	Sequence
Linearisation of pCDF DUET-1 MCS-1 slot fwrd	TCTACTAGCGCAGCTTAA
Linearisation of pCDF DUET-1 MCS-1 slot rev	TATGTATATCTCCTTCTTATACTT
Cloning of a gene from pET28a into MCS-1 of pCDF-DUET-1 fwrd	CTTTCTGTTCGACTTGCCGGATCTCAGTGGTGG
Cloning of a gene from pET28a into MCS-1 of pCDF-DUET-1 rev	AAGGAGATATACCATAAGAAGGAGATATACCATGGGCAGC
Cloning of ecmetF or mmsahh behind rganmt in pET28a after digestion with XhoI fwrd	GGTGGTGGTGCTCGATTTGTTAGCAGCCGGATCTCAG
Cloning of ecmetF or mmsahh behind rganmt in pET28a after digestion with XhoI rev	TGCGGCCGCACTCGATTAAGAAGGAGATATACCATGGGC
Constitutive promoter Ppgi-mut from pFCM [40]	GTGGAATTATAGCATTTTAGCCTTTAATTGTCAATAGGTCTCGAGGTGAAGACGAAAGGGCCTCG
Constitutive promoter PSTRONG from pDM4:SS9-C1M [13,53,58]	AATACTTGACATATCACTGTGATTCACATATAATATGCG
RBSC [53]	AAGTTAAGAGGCAAGA