Figure 4.

UBvR-mediated antigen presentation relies on mitochondrial-derived ROS

(A) Flow cytometry analysis of mitochondrial-derived ROS using MitoSOX staining.

(B) MFI analysis of the signal obtained in (A).

(C) Flow cytometry analysis of cytoplasmic ROS using DHE staining.

(D) MFI analysis of the signal obtained in (C).

(E) Antigen presentation assay using the different MSC populations following their treatment with anti-oxidants. The color codes are as follow: Ctl MSCs (black); MSC-OVA (green); MSC-RNP-OVA (blue); and MSC-UBvR-OVA (red). The different treatment groups are non-treated cells (NT), treatment with N-acetylcysteine (NAC), treatment with MitoTEMPO (MiTO); treatment with α-tocopherol (AT).

(F) ELISA-based quantification of the OVA protein in the supernatant of MSC-OVA or MSC-UBvR-OVA in the presence or absence of MitoTEMPO.

For this panel, n = 5/group with ∗∗∗p < 0.001. Data are represented as mean ± SD.