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. 2024 Oct 24;27(3):695–710. doi: 10.1093/neuonc/noae224

Figure 5.

Figure 5.

c-Fos overexpression rescues the effects caused by ROR1 knockdown. (A) Sphereformation analysis of T3359 and T456 GSCs with ROR1 knockdown, with/without FOS overexpression. Scale bar, 200 μm. (B) The quantification of relative sphere number in (A). (C) Cell viability assay of T3359 and T456 GSCs with ROR1 knockdown, with/without FOS overexpression. (D) H&E staining of T3691 GSCs-derived tumors 25 days after transplantation. GSCs with ROR1 knockdown and with/without lentivirus-mediated FOS overexpression were used for intracranial injection. Areas with hyperchromatic nuclei are tumors. Scale Bar, 200 μm. (E) Western blot analysis of ROR1, c-Fos, cell cycle regulators (CCND1, CDK2, and CDK6), and apoptosis markers (Cleaved PARP and Cleaved Caspase3) in T3691 and T456 GSCs with ROR1 knockdown and with/without lentivirus-mediated FOS overexpression. (F) Kaplan–Meier survival curve of mice injected with T3691 GSCs with ROR1 knockdown and with/without lentivirus-mediated FOS overexpression (n = 5 per group). (G) Immunofluorescent co-staining of ROR1 and Ki67, Cleaved Caspase3 in T3691 GSCs-derived xenografts with ROR1 knockdown and with/without lentivirus-mediated FOS overexpression. Scale Bar, 25 μm. (H) Statistical analysis of Ki67 and Cleaved Caspase3 positive staining in xenografts of (G). *P < .05; **P < .01; ***P < .001; ****P < .0001. Data are represented as mean ± SEM.