Table 2.

Primer and probe concentration optimization

target	Mean CT in 1.5,1.5,1 pM Primer F, Primer R, probe concentration	Mean CT in 3,3,1 pM Primer F, Primer R, probe concentration	Mean CT in 6,6,2 pM Primer F, Primer R, probe concentration
HPV 16	24.37	21.97	22.46
HPV 18	27.14	25.39	26.08
HPV 31	22.70	21.78	22.89
HPV 33	24.43	24.67	25.47
HPV 35	26.97	26.12	28.31
HPV 39	28.69	25.32	25.19
HPV 45	22.62	20.74	23.47
HPV 51	20.86	21.24	22.75
HPV 52	30.11	28.67	29.39
HPV 56	24.01	22.49	24.41
HPV 58	22.98	19.81	20.82
HPV 59	27.70	26.35	25.73
HPV 66	27.85	27.09	27.44
HPV 67	24.67	23.16	23.48
HPV 68	20.52	17.21	19.34
β Globin	20.41	16.55	16.28