Figure 3. Immunoblots of Liver Homogenates from MCAD^+/+ and MCAD^−/− Mice.

These were probed with anti-MCAD antibody or anti-SCAD antibody. Homozygous MCAD^−/− mice had no detectable MCAD protein. MCAD protein is only detectable under the MCAD protein–spiked (positive control) lane. As a control analysis, liver homogenates probed with anti-SCAD antibody revealed that SCAD protein was present in both MCAD^+/+ and MCAD^−/− mice. No MCAD positive-control protein is detected by anti-SCAD antibodies (MCAD lane). mw, molecular weight standards.