Fig. 6.
HM prohibited BoNT/AHCC from binding to SV2C. Molecular level: (A) SPR analysis HM competed with SV2C to bind to AHCc. SV2C was immobilized on the CM5 chip. Red: AHCC (400 nM) + HM (0 nM), green: AHCC (400 nM) + HM (31.25 nM), blue: AHCC (400 nM) + HM (62.5 nM), Purple: AHCC (400 nM) + HM (125 nM). The signal (Ru) was diminished with the increasing concentration of HM. Cell level: Cell fluorography showed the blockade of AHCc binding to cells by HM. Sample concentration: AHCC-Alexa Flour 488 (10 μg/mL), HM (10 μg/mL, 50 μg/mL, 100 μg/mL). (B) The relative fluorescence intensity of cells was decreased by HM. (C) The cell fluorescence intensity diminishes as the concentration of HM increases.(For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)