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. Author manuscript; available in PMC: 2025 Sep 2.
Published in final edited form as: Mol Pharm. 2024 Aug 20;21(9):4324–4335. doi: 10.1021/acs.molpharmaceut.4c00298

Figure 2.

Figure 2.

Conjugation and radiolabeling of EVs for PET. (a) Schematic showing the conjugation of the chelator (Df) to the surface of EVs. (b) Nanoparticle tracking analysis showed no differences in the size distribution of MSC EVs and Df-MSC EVs after conjugation. (c) Representative instant thin layer chromatography used to determine the (d) radiolabeling efficiency, which exceeded 85% for all radiolabeled EVs (n=3). (e) Stability studies of 89Zr-Df-MSC EVs showing that radiolabeling was stable in PBS and 50% mouse serum up to 72 h following incubation (n=3).