Table 2.

Summary of AAV delivery by FUS-BBBO in mice, rats, and NHPs.

Animal		AAV parameters			FUS parameters						Ref
Animal	Target	Serotype	Gene	Dose (vg/g)	Microbubble (MB) Dose	Frequency (MHZ)	Pressure (MPa)	PRF (Hz)	Pulse length (ms)	Duration (s)
Mouse	CT, STR, HP, TH	AAV9	GFP	5×108 2.5×109 1.25×1010	40–80 μL/kg	0.558 1.18	0.3 0.53–0.6	1	10	120	[130]
	HP, CT	AAV9	mcherry-ChR2	4×1011 vg/animal	5 μL	1.5	0.74	5	10	120	[145]
	HP, SN, OB, DMV	AAV9		1.25×1010	0.02 mL/kg	1.68	1.0 ± 0.2 1.1 ± 0.2	1	10	120	[146]
	STR, HP, midbrain	AAV9		6.7×109 1.3×109	1.5×106 MB	1.5	0.33	1	10	120	[147]
	Whole brain	AAV9	GFP	1.1×1011 vg/animal	8 μL/mL at 100 μL volume	1.5	0.57	10	99	150	[148]
	HP	AAV9	GFP	6.5×1010 1.0×1010 1.3×1011 vg/animal	3.6×108 MBs/mL	1.5	1.0	0.33	1.0 3.33 6.67	120	[149]
	STR, TH	AAV9	GFP	1×1011 1.3×1010	0.02 mL/kg	1.78	0.06 0.12 0.25 0.38	0.5	10	120	[150]
	Large brain regions	AAV9	GFP	1×1010	1.5×106 MBs	1.53	0.27	1	10	30	[151]
	HP	AAV9-hM4Di	mcherry	1×1010	1.5×106 MBs	1.5	0.42	1	10	120	[152]
	CT	AAV9, PHP.B		1×1011 vg/animal	1.5×107 MBs in 50 μL volume	1.5	0.51	5	1.0	660	[153]
	CT, STR, TH	AAV9, PHP.B	GFP	1×1010 1×1011	0.02 mL/kg	1.68 to 1.78	~0.5 – 2.0	1	10	120	[139]
	HP	AAV9 AAV1	mcherry-ChR2	2.48×1012 vg/animal	~2.5×107 MBs	1.5	0.45	5	6.7	60	[154]
	STR, TH	AAV9 AAV2-HBKO	GFP	1.7×109 3.3×109 1.7×1010	0.02 mL/kg	0.58	NA	1	10	120	[155]
	STR, HP, TH, CT	AAV6 AAV1/2 AAV9	GFP	3×109	0.02 mL/kg	1.68	NA	1	10	120	[156]
	CT, STR, TH, HP	AAV1, 2, 5, 8, 9, rg	TdTomatoGFP	3.33×109 1.67×1010	0.02 mL/kg	0.58	<0.9	1	10	180	[131]
	STR	AAV2	hrGFP	1×109	0.3 mL/kg	1.5	0.44 0.53 0.7	1	10	120	[136]
	CPu	AAV1	GFP	1.1×1011 vg/animal	~2.5×107 MBs	1.5	0.45	5	20	300	[138]
	CT, HP	AAV1/2	GFP	3×109	0.02 mL/kg	1.68	NA	1	10	120	[157]
	HP	AAV9-Cu64	PKM2	2×1011 5×1011 vg/animal	2.5×108 MBs/kg	1.5	0.42 0.6 0.74	NA	NA	120	[158]
Rat	Left brain	AAV2/1	LacZ	1.06×1011 vg/animal	0.2×109 MBs/mL	0.5	1.1	1	10	40+	[159]
	STR	AAV1/2	GFP	1×109	0.4×108 − 0.64×108 MBs/kg		0.97	1	10	200	[137]
	STR, SN	AAV2	NA	2.96×1012 − 3.94×1012 vg/animal	NA	0.58	0.4	1	10	120	[160]
NHP	CT, TH, ST midbrain	AAV9-PHP.eB AAV9.2-PHP.eB	GFP	5×1013 vg/animal	4 μL/kg	0.220	1 Watts	NA	NA	60 to 150	[143]
	CT	AAV2 AAV9	EGFP mCherry	NA	200 μL/kg	1.46	1.17	1	10	60	[144]

CT: Cortex; STR: Striatum; HP: Hippocampus; TH: Thalamus; CPu: Caudate putamen; Cd: Caudate nucleus; SN: Substantia nigra; OB: Olfactory bulb; DMV: Dorsal motor nucleus of the vagus; STN: Subthalamic nucleus; NHP: Non-human primates.