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2FDU to 2FDA conversion titer of NDT colonies identified through screening
| Colony | Nucleotide change | Amino acid change | Yield (Ratio of 2FDA to fNDT 2FDA area) |
|---|---|---|---|
| NDT | – | – | 0.42 |
| fNDT | T176A | L59Q | 1 |
| fNDT-i1 | T155C, T176A, T180C, T264C | V52A, L59Q | 1.88 |
| fNDT-i2 | C82A, T176A | L28I, L59Q | 1.24 |
| fNDT-i4 | C82A, T155C, T176A | L28I, V52A, L59Q | 1.96 |
